Literature summary extracted from

Rodriguez-Lopez, A.; Almeciga-Diaz, C.J.; Sanchez, J.; Moreno, J.; Beltran, L.; Diaz, D.; Pardo, A.; Ramirez, A.M.; Espejo-Mojica, A.J.; Pimentel, L.; Barrera, L.A.
Recombinant human N-acetylgalactosamine-6-sulfate sulfatase (GALNS) produced in the methylotrophic yeast Pichia pastoris (2016), Sci. Rep., 6, 29329 .

Application

EC Number	Application	Comment	Organism
3.1.6.4	synthesis	removal of the native signal peptide and coexpression with human formylglycine-generating enzyme SUMF1 in Pichia pastoris allow an improvement of 4.5fold in the specific GALNS activity. Recombinant GALNS shows a high stability at 4°C, while the activity is markedly reduced at 37 and 45°C. Recombinant GALNS is taken-up by HEK-293 cells and human skin fibroblasts in a dose-dependent manner, without any additional protein or host modification	Homo sapiens

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
3.1.6.4	expression in Pichia pastoris	Homo sapiens

Organism

EC Number	Organism	UniProt	Comment	Textmining
3.1.6.4	Homo sapiens	P34059	-	-

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Release 2023.1 (January 2023)